- Catalog No: MBE-0011
- Species: Human
- Source: ExpiSf9™ Cells
- Substrates: UK-157147 or others
- Overview:
- As a biology or pharmacology expert, I am delighted to introduce the uridine diphosphate glucuronosyltransferase 2B15 (UGT2B15) enzyme, a member of the extensive UGT superfamily that plays a pivotal role in the metabolism of a diverse array of compounds, including drugs, hormones, and other endogenous and exogenous substances.
Basic Information
Full Name: UDP Glucuronosyltransferase 2 Family, Polypeptide B15 (UGT2B15)
Distribution: Primarily localized in the endoplasmic reticulum, microsomes, membranes, and membrane-bound compartments.
Activity: Functions as a glucuronosyltransferase, catalyzing the transfer of glucuronic acid from uridine diphosphate glucuronic acid (UDPGA) to various substrates
Key Functions in Drug Metabolism
Drug Glucuronidation:
UGT2B15 is involved in the glucuronidation of numerous drugs, a phase II metabolic reaction that enhances the water solubility and facilitates the excretion of lipophilic compounds. This process is crucial for the elimination of drugs and their metabolites from the body.
Metabolic Pathway Diversity:
Along with other UGT enzymes, UGT2B15 contributes to the complex metabolic network that determines the fate of drugs within the body. Its specific substrate profile makes it a key player in the metabolism of certain drug classes.
- Application in Drug Development:
- Drug Metabolism Prediction and Optimization:
- Understanding the role of UGT2B15 in drug metabolism is essential for predicting the pharmacokinetic behavior of drug candidates. Researchers utilize in vitro and in vivo models to study the interaction between UGT2B15 and potential drugs, informing decisions on drug design, dosing, and administration.
- Toxicity Assessment and Mitigation:
- High-Throughput Screening Platforms:
- Given its involvement in the metabolism of multiple drugs, UGT2B15 can mediate drug-drug interactions. By studying the potential for UGT2B15 inhibition or induction by drug candidates, researchers can identify potential adverse effects and develop strategies to mitigate them.
- In Vitro and In Vivo Models:
- UGT2B15 recombinant enzymes and in vitro assay systems are valuable tools for screening drug candidates and evaluating their metabolic stability and potential for drug-drug interactions. These systems enable researchers to rapidly assess the potential impact of UGT2B15 on drug metabolism, guiding the development of safer and more effective drugs.
- Appearance: Lyophilized powder.
- Endotoxin Level: <1.0 EU/μg, determined by LAL method.
- Purity: Greater than 90% as determined by reducing SDS-PAGE.
- Storage & Stability: Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
